Journal: Journal of Experimental Pharmacology

Article Title: Pharmacological Activity of Chemical Compounds of Potato Peel Waste ( Solanum tuberosum L.) in vitro: A Scoping Review

doi: 10.2147/JEP.S435734

Figure Lengend Snippet: Characteristic Distribution of Articles on Potato Peel

Article Snippet: 6 , Helal et al, 2020. Egypt , Evaluation of potato peel extract as a source of anti-oxidant and antimicrobial substances , Solanum tuberosum , Water, methanol, and ethanol (80 and 100%) extracts from potato peels , Phenol and flavonoid compounds (vanillic and hesperidin) , No (anti-microbial and anti-oxidant activity) , Anti-oxidant activity with DPPH and anti-microbial with Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 29213, E. coli ATCC 25922, Salmonella t. ATCC14028, and C. albicans ATCC 10231 , Dose 70 μL (200 and 400 ppm), potato peel methanol extract 400 ppm was more effective in inhibiting gram-positive, gram-negative bacteria and Candida albicans than ampicillin (control).

Techniques: Variant Assay, Activity Assay, In Vitro, Antioxidant Activity Assay, Biomarker Discovery, Bacteria, Control, Concentration Assay, Expressing, Protein-Protein interactions

Journal: Brazilian Journal of Microbiology

Article Title: Antimicrobial potential of pyroligneous extracts – a systematic review and technological prospecting

doi: 10.1016/j.bjm.2018.07.001

Figure Lengend Snippet: Demographic data from articles and patents considered in this study.

Article Snippet: , Salmonella enterica serovar typhimurium (ATCC #14028) ( S. typhimurium) American Type Tissue Culture Collection (ATCC, Manassas, Va., U.S.A.). , Rice ( Oryza sativa L.) hull. , Concentrations of 0.1%, 0.5%, and 1.0% (v/v). , Disk diffusion method. , n = 3 , 24 h , Not informed. , Not informed. , As antimicrobial flavor formulations for application to human foods and animal feeds..

Techniques: Positive Control, Negative Control, Sterility, Control, Virus, Isolation, Infection, Diffusion-based Assay, Time-Kill Assay, Concentration Assay, Bacteria, In Vitro, In Vivo

Journal: Cell reports

Article Title: In situ chemoproteomic profiling reveals itaconate inhibits de novo purine biosynthesis in pathogens.

doi: 10.1016/j.celrep.2024.114737

Figure Lengend Snippet: Figure 1. Quantitative chemoproteomic profiling of S-itaconations in living S. Typhimurium SL1344 (A) Chemical structure of the C3A probe (top) and covalent modification of cysteine residues by itaconate to form ‘‘S-itaconation.’’ (B) Itaconate competes with C3A labeling in living S. Typhimurium SL1344. Bacteria are preincubated with different concentrations of itaconate (10, 20, and 30 mM) for 1 h followed by C3A probe labeling (30 mM, 2 h). The probe-labeled samples are reacted with azide-TAMRA by click chemistry and visualized by in-gel fluorescence; Coomassie brilliant blue (CBB) staining demonstrates equal loading. (C) Comparison of identified itaconated sites in S. Typhimurium SL1344 between the current in situ labeling vs. the previous in vitro labeling (Zhang et al.21), and their overlap is shown in Venn diagrams. Bacteria are preincubated with itaconate (20 mM, 1 h) followed by the C3A labeling (30 mM, 2 h). (D) Volcano plot of the quantified C3A-captured sites that are competed by itaconate in living S. Typhimurium SL1344. The thresholds for both the itaconate competition ratio and -log10(q value) are set as 1.5. The orange dots indicate sites that are significantly competed by itaconate from n = 4 biological repeats, and the green dots represent proteins from the de novo purine biosynthesis pathway. (E) Gene Ontology analysis for itaconated sites from the in situ profiling. The pathways related to purine metabolism are significantly enriched.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Casamino acids VWR Cat# J851-100G Succinate Sigma-Aldrich Cat# S3674 Malate Sigma-Aldrich Cat# 02288 Fumarate Sigma-Aldrich Cat# 47910 AMP Sigma-Aldrich Cat# A2252 Fetal bovine serum (FBS) Gibco Cat# 10099-141C Penicillin and streptomycin (P/S) Gibco Cat# 15140-122 Dulbecco’s modified Eagle’s medium (DMEM) Gibco Cat# C11995500CP Opti-MEMTM I Reduced Serum Medium Gibco Cat# 31985070 Gentamicin Sigma-Aldrich Cat# G1264 PageRulerTM Prestained Protein Ladder, 10 to 180 kDa Thermo Fisher Scientific Cat# 26616 Precision Plus Protein Dual Color Standards Bio-Rad Cat# 1610374 Critical commercial assays PierceTM BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 KOD OneTM PCR Master Mix -Blue- TOYOBO Cat# KMM-201 KOD -Plus- Mutagenesis Kit TOYOBO Cat# SMK-101 Glutamate-GloTM Assay Promega Cat# J7021 NAD/NADH-GloTM Assay Promega Cat# G9071 QIAGEN Plasmid Mini Kit QIAGEN Cat#12125 HiPure Gel Pure Micro Kit Magen Cat# D2110-02 ClonExpress II One Step Cloning Kit Vazyme Cat# C112-02 X-tremeGENETM 9 Roche Cat# 6365779001 PierceTM ECL Western Blotting Substrate Thermo Fisher Scientific Cat# 32209 Immobilon Western Chemiluminescent HRP Substrate Millipore Cat# WBKLS0100 Deposited data MS Raw data iProx PXD050510 Experimental models: cell lines Mouse: Raw264.7 ATCC Cat#TIB-71 Mouse: Raw264.7 IRG1 / This study N/A Experimental models: organisms/strains Staphylococcus aureus FPR3757 Renji Hospital, School of Medicine, Shanghai Jiaotong University N/A Pseudomonas aeruginosa PAO1 University of Chinese Academy of Sciences N/A Salmonella Typhimurium SL1344 ATCC Cat# 14028 Salmonella Typhimurium SL1344 DguaB This study N/A Salmonella Typhimurium SL1344 DpurF This study N/A Salmonella Typhimurium SL1344 DICL This study N/A Oligonucleotides oligonucleotides This study Table S4 Recombinant DNA Plasmid: pET21b-guaB-His6 This study N/A Plasmid: pET21b-guaB-His6 C305A This study N/A Plasmid: pET21b-guaC-His6 This study N/A Plasmid: pET21b-guaC-His6 C186A This study N/A Plasmid: pET21b-guaA-His6 This study N/A (Continued on next page) 12 Cell Reports 43, 114737, September 24, 2024

Techniques: Labeling, Bacteria, Staining, Comparison, In Situ, In Vitro